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Compugen Inc compugen zebrafish array
Comparison between RT-PCR and <t>microarray</t> results for selected genes. Real-time PCR was done for 16 clones from Cluster 2 using the same RNA used for array hybridization. The RT-PCR profile closely parallels the microarray data, cross-validating both techniques as quantitative estimates.
Compugen Zebrafish Array, supplied by Compugen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Comparison between RT-PCR and microarray results for selected genes. Real-time PCR was done for 16 clones from Cluster 2 using the same RNA used for array hybridization. The RT-PCR profile closely parallels the microarray data, cross-validating both techniques as quantitative estimates.

Journal: PLoS Genetics

Article Title: Transcriptome Analysis of Zebrafish Embryogenesis Using Microarrays

doi: 10.1371/journal.pgen.0010029

Figure Lengend Snippet: Comparison between RT-PCR and microarray results for selected genes. Real-time PCR was done for 16 clones from Cluster 2 using the same RNA used for array hybridization. The RT-PCR profile closely parallels the microarray data, cross-validating both techniques as quantitative estimates.

Article Snippet: The annotation for all the GenBank entries in the Compugen array is available from the GIS-sponsored Web site designed for zebrafish microarray annotation ( http://giscompute.gis.a-star.edu.sg/~govind/zebrafish/index.html ).

Techniques: Comparison, Reverse Transcription Polymerase Chain Reaction, Microarray, Real-time Polymerase Chain Reaction, Clone Assay, Hybridization

All the clones subjected for in situ analysis showed a lower intensity of hybridization signal at one- to four-cell stages and increased intensity at 64-/128-cell and 256-/512-cell stages. These observations support the data obtained in the real-time PCR and microarray data, indicating the pre-MBT accumulation of transcripts.

Journal: PLoS Genetics

Article Title: Transcriptome Analysis of Zebrafish Embryogenesis Using Microarrays

doi: 10.1371/journal.pgen.0010029

Figure Lengend Snippet: All the clones subjected for in situ analysis showed a lower intensity of hybridization signal at one- to four-cell stages and increased intensity at 64-/128-cell and 256-/512-cell stages. These observations support the data obtained in the real-time PCR and microarray data, indicating the pre-MBT accumulation of transcripts.

Article Snippet: The annotation for all the GenBank entries in the Compugen array is available from the GIS-sponsored Web site designed for zebrafish microarray annotation ( http://giscompute.gis.a-star.edu.sg/~govind/zebrafish/index.html ).

Techniques: Clone Assay, In Situ, Hybridization, Real-time Polymerase Chain Reaction, Microarray